Ex vivo Lung Perfusion: A Technique for Pulmonary Research in a Rabbit Model

Published: April 30, 2023

Abstract

Source: Pacheco-Baltazar, A., et al., Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021).

This video shows an ex vivo technique for isolated lung perfusion. It is a tool for pulmonary research that will aid studies of various physiological and pathological mechanisms involved in lung transplantation procedures.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Cannulation: Place the freshly isolated rabbit's lungs along with heart over a sterile gauze on a petri dish. To prevent atelectasis, ventilate the lungs using positive-pressure ventilation with positive end-expiratory pressure (PEEP) set at 2 cmH2O. Remove the ventricles by cutting them off the heart …

Divulgations

The authors have nothing to disclose.

Materials

2-Stop Tygon E-Lab Tubing, 3.17 mm ID, 12/pack, Black/White Hugo Sachs Elektronik (HSE) 73-1864
Adapter for Positive Pressure Ventilation on IPL-4 Hugo Sachs Elektronik (HSE) 73-4312
Adapter for Positive Pressure Ventilation on IPL-4 Hugo Sachs Elektronik (HSE) 73-4312
Alternative Pressure-Free Gas Supply for IPL-4: To supply the trachea with gas mixture different from room air during negative ventilation Hugo Sachs Elektronik (HSE) 73-4309
Base Unit for the Rabbit to Fetal Pig Isolated Perfused Lung Hugo Sachs Elektronik (HSE) 73-4138
Bovine serum A2:D41albumin lyophilized powder sigma 3912 500 g
Calcium chloride, CaCl2·2H2O. JT Baker 10035-04-8
Cryogenic vials Corning 430659 2 mL
D-glucose, C6H12O6. sigma G5767
Differential Low Pressure Transducer DLP2.5, Range +- 2.5 cmH2O, HSE Connector Hugo Sachs Elektronik (HSE) 73-3882
Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector Hugo Sachs Elektronik (HSE) 73-0064
Eppendorf tubes
Ethanol absolute HPLC grade Caledon
Falcon tubes 14 mL
Harvard Peristaltic Pump P-230 (Complete with Control Box and P-230 Motor Drive) Hugo Sachs Elektronik (HSE) 70-7001
Heated Linear Pneumotachometer 0 to 10 L/min flow range Hugo Sachs Elektronik (HSE) 59-9349
Heater Controller for Single Pneumotachometer 230 VAC, 50 Hz Hugo Sachs Elektronik (HSE) 59-9703
Heparin PISA 5000 UI
HPLC Column (C18 100A 5U) Alltech 98121213 150 mm x 4.6 mm
Hydrophilic Syringe Filter Millex SLLGR04NL 4 mm
IPL-4 Core System for Isolated Rabbit to Fetal Pig Lung, 230 Hugo Sachs Elektronik (HSE) 73-4296
IPL-4 Core System for Isolated Rabbit to Fetal Pig Lung, 230 V Hugo Sachs Elektronik (HSE) 73-4296
Jacketed Glass Reservoir for Buffer Solution, with Frit and Tubing, 6.0 L Hugo Sachs Elektronik (HSE) 73-0322
Lauda Thermostatic Circulator, Type E-103, 230 V/50 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C Hugo Sachs Elektronik (HSE) 73-0125
Left Atrium Cannula for Rabbit with Basket, OD 5.9 mm Hugo Sachs Elektronik (HSE) 73-4162
Low Range Blood Pressure Transducer P75 for PLUGSYS Module Hugo Sachs Elektronik (HSE) 73-0020
Magnesium sulfate heptahydrate, MgSO4·7H2O JT Baker 10034-99-8
Microcentrifuge Tube Corning 430909
Negative Pressure Ventilation Control Option with Pressure Regulator for IPL-4 Hugo Sachs Elektronik (HSE) 73-4298
New Zeland rabbits
PISABENTAL (Pentobarbital sodium) PISA Q-7833-215
PLUGSYS Case, Type 603* 7 Hugo Sachs Elektronik (HSE) 73-0045
PLUGSYS TCM Time Counter Module Hugo Sachs Elektronik (HSE) 73-1750
PLUGSYS Transducer Amplifier Module (TAM-A) Hugo Sachs Elektronik (HSE) 73-0065
PLUGSYS Transducer Amplifier Module (TAM-D) Hugo Sachs Elektronik (HSE) 73-1793
PLUGSYS VCM-4R Ventilation Control Module with Pressure Regulator Hugo Sachs Elektronik (HSE) 73-1755
Potassium chloride, KCl. JT Baker 3040-01
Potassium dihydrogen phosphate, KH2PO4 JT Baker 7778-77-0
Pulmonary Artery Cannula for Rabbit with Basket, OD 4.6 mm Hugo Sachs Elektronik (HSE) 73-4161
Servo Controller for Perfusion (SCP Hugo Sachs Elektronik (HSE) 73-2806
Snap Cap Microcentrifuge Tube Costar 3620 1.7 mL
Sodium bicarbonate, NaHCO3 sigma S6014
Sodium chloride, NaCl. sigma S9888
Surgical gloves No. 7 1/2
Surgical gloves No. 8
Taygon tubes Masterflex
Tracheal Cannula for Rabbit, OD 5.0 mm Hugo Sachs Elektronik (HSE) 73-4163

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Citer Cet Article
Ex vivo Lung Perfusion: A Technique for Pulmonary Research in a Rabbit Model. J. Vis. Exp. (Pending Publication), e20820, doi: (2023).

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