Mouse Antral Oocyte Isolation: A Method to Isolate Antral Oocytes from Freshly Harvested Mouse Ovaries
Published: April 30, 2023
Abstract
Source: Monti, M. et al. Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization. J. Vis. Exp. (2016)
This video describes the isolation of antral oocytes from freshly harvested mouse ovaries. These antral oocytes can be used for further characterization and developmental studies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animals Maintain adult 3- to 6-week-old female mice in a room with controlled temperature and humidity (with phases of 12L:12D and fed ad libitum). Inject mice intraperitoneally with 2.5 IU of Pregnant mare serum gonadotropin (PMSG) to stimulate follicular growth by mimicking the effects of folli…
Representative Results
Figure 1. Preparation of a mouth pipette using glass Pasteur pipettes and a Bunsen burner. Figure 2. Intact ovaries in Petri dish filled with pre-warmed and equilibrated M2 medium.</stron…
Divulgations
The authors have nothing to disclose.
Materials
| PMSG | Sigma | G4527 | |
| EmbrioMax M2 | Millipore | MR-015-PD | |
| Mineral Oil | Sigma | M8410 | |
| Cell Culture Dish 35 mm x 10 mm | Corning | 430165 | |
| µ-Dish 35 mm, high glass bottom | Ibidi | 81158 | |
| Pipette Pasteur Borosilicate | Corning | 7095D-9 | |
| Aspirator tube assemblies for calibrated micropillary pipettes | Sigma | A5177 |
Tags
Citer Cet Article
Mouse Antral Oocyte Isolation: A Method to Isolate Antral Oocytes from Freshly Harvested Mouse Ovaries. J. Vis. Exp. (Pending Publication), e20757, doi: (2023).