Source: Monti, M. et al. Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization. J. Vis. Exp. (2016)
This video describes the isolation of antral oocytes from freshly harvested mouse ovaries. These antral oocytes can be used for further characterization and developmental studies.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Animals
2. Hormone Preparation
3. Antral Oocytes Isolation
For proper oocytes isolation:
Figure 1. Preparation of a mouth pipette using glass Pasteur pipettes and a Bunsen burner.
Figure 2. Intact ovaries in Petri dish filled with pre-warmed and equilibrated M2 medium.
Figure 3. Initial isolation of antral oocytes from antral follicles by means of a sterile needle.
Figure 4. Transfer of the freshly isolated oocytes with a mouth pipette from the Petri dish (A) to a different Petri dish containing small drops of pre-warmed and equilibrated M2 medium (B).
The authors have nothing to disclose.
PMSG | Sigma | G4527 | |
EmbrioMax M2 | Millipore | MR-015-PD | |
Mineral Oil | Sigma | M8410 | |
Cell Culture Dish 35 mm x 10 mm | Corning | 430165 | |
µ-Dish 35 mm, high glass bottom | Ibidi | 81158 | |
Pipette Pasteur Borosilicate | Corning | 7095D-9 | |
Aspirator tube assemblies for calibrated micropillary pipettes | Sigma | A5177 |