Source: Chai, M. G., et al. Bioluminescent Orthotopic Model of Pancreatic Cancer Progression. J. Vis. Exp. (2013).
This video demonstrates an orthotopic model of pancreatic cancer that utilizes Matrigel for localized cell delivery and in vivo bioluminescence imaging for non-invasive monitoring of tumor progression. The orthotopic model is suited to both syngeneic and xenograft models and could be used in pre-clinical trials to investigate the impact of novel anti-cancer therapeutics on the growth of the primary pancreatic tumor and metastasis.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Transducing Pancreatic Cancer Cell Lines
2. Pancreatic Cancer Cell Preparation
3. Mouse Preparation
4. Laparotomy
5. Abdominal Wall Closure
6. Bioluminescent Tracking of Pancreatic Cancer Progression
The authors have nothing to disclose.
Clean Bench coat | |||
Heating pad | Set to 37 °C | ||
Ivis Lumina ll Bioluminescent imager | Caliper | Alternative bioluminescent imaging systems include In vivo F PRO (Carestream) and Photon Imager (Biospace Lab) | |
Dissecting scissors | |||
Iris forceps (serrated) | |||
Needle holder | |||
27 G 0.3 ml insulin syringe | Terumo | T35525M2913 |