Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model
Published: April 30, 2023
Abstract
Source: Wang L. et. al., Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment. J. Vis. Exp. (2019).
This video describes the technique to create a zebrafish patient derived xenograft model. In the sample protocol, we assess the efficiency of the drugs Gemcitabine and Navitoclax on the pancreatic tumor xenograft model.
Protocol
1. Injecting Mixed Cell Suspension into the Zebrafish Add 10x tricaine solution into E3 water to anesthetize zebrafish larvae and transfer the larvae (from step 2.3) to the injection plate filled by modified E3 (E3 with 1 g/L glucose and 5 mmol/L L-glutamine). Fill 25 µL of mixed cell suspension into micro capillaries needle and insert the needle into the micro-injection manipulator. Set injection pressure and time. Inject 50-80 cells (~8 nL) into the yolk sac of 48 h…
Materials
| L-glutamine | GIBCO | 21051024 | |
| Gemcitabine | Gemzan | ||
| Methylcellulose | Sigma | M0262 | |
| Navitoclax(ABT-263) | Selleck | S1001 | Bcl-xL inhibitor |
| Equipment | |||
| Microinjector | NARISHIGE | ||
| Confocal Microscope | LEICA | SP8 | 0.00 |
Citer Cet Article
Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model. J. Vis. Exp. (Pending Publication), e20198, doi: (2023).