This video describes a method to dissociate whole worms into a single-cell suspension suitable for FACS or immunoprecipitation of intact cells.
Protocol
This protocol is an excerpt from Germany et al, Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans, J. Vis. Exp. (2019). 1. Preparation and collection of aged worms for cell isolation NOTE: Explained below is the isolation of cholinergic neurons…