Source: Escorcia, W., et al. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining. J. Vis. Exp. (2018).
This video describes a protocol to stain lipids in whole, fixed worms using Nile Red dye. To specifically view the neutral lipids in the core of lipid droplets, image the green emission spectra of the stained C. elegans.
This protocol is an excerpt from Escorcia et al, Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining, J. Vis. Exp. (2018).
1. Nile Red (NR) Staining of Lipids
Imager.M2m Microscope | Zeiss | n/a | Fluorescence microscope |
ERC5s camera | Axiocam | n/a | Color-capable |
MRm camera | Axiocam | n/a | Fluorescence-capable |
Nile red | Thermo Fisher | N1142 | Lipid Stain |
Isopropyl Alcohol | BDH | BDH1133-1LP | Fixative solution |
Centrifuge 5430 | Eppendorf | 5428000015 | Centrifuge |
Tube Rotator | VWR | 10136-084 | Rotator |