Source: Gervaise, A. L. and Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (2016).
This video describes a method to dissect gonads from adult C. elegans hermaphrodites, resulting in tissues that are suitable for immunostaining and fluorescent imaging.
This protocol is an excerpt from Gervaise and Arur, Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (2016).
1. Dissection of Adult Worms for Obtaining Gonads
Figure 1: Demonstration of Needle Positions during dissections. Left: Photograph of a dissection in process. Right: Needle positions with reference to the worm. Please click here to view a larger version of this figure.
Flat bottom glass watch dish | Agar Scientific | AGL4161 | We use glass because dissected gonads often stick to plastic |
25 G needles | BD PrecisionGlide | 305122 | |
Syringes (could be 1 or 5 mL) | BD Syringes | 1 mL: BD 309659 | |
Microscope slides (25 x 75 x 1.0 mm) | Fisherbrand | 12-550-343 | |
Clinical bench top centrifuge | |||
*M9 solution | |||
Levamisole | Sigma | L9756 | |
*M9 Buffer Recipe | 3 g KH2PO4, 6 g Na2HPO4, 5 g NaCl, 1 mL 1 M MgSO4, H2O to 1 L. |