JOVE JOURNAL

Immunologie et infection

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Journal (Immunology and Infection)

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Travis J. Chiarelli¹, Nicole A. Grieshaber¹, Scott S. Grieshaber¹

¹Department of Biological Sciences,University of Idaho

Journal (Immunology and Infection)

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Xiaoyue Sun*^1,2,3, Xiaoqiang Yang*^1,2,3,4, Chui Zeng^1,2,3, Fayed Attia Koutb Megahed^1,2,3,5, Qi Zhou^1,2,3, Tingdang Liu^1,2,3, Waqas Iqbal^1,2,3, Pingnan Sun^1,2,3, Xiaoling Zhou^1,2,3

¹Stem Cell Research Center,Shantou University Medical College, ²The Center for Reproductive Medicine,Shantou University Medical College, ³Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College, ⁴Medical Research Center, Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University, ⁵Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute,General Autority - City of Scientific Researches and Technological Applications

Journal (Immunology and Infection)

Vector Competence Analyses on Aedes aegypti Mosquitoes using Zika Virus

Sasha R. Azar^1,2, Scott C. Weaver^1,3,4

¹Institute for Human Infections and Immunity,University of Texas Medical Branch, ²Department of Pathology,University of Texas Medical Branch, ³Department of Microbiology and Immunology,University of Texas Medical Branch, ⁴World Reference Center for Emerging Viruses and Arboviruses,University of Texas Medical Branch

Journal (Immunology and Infection)

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Aditi Upadhye¹, Melissa Marshall², James C. Garmey², Timothy P. Bender³, Coleen McNamara²

¹Department of Microbiology, Immunology, Cancer Biology,University of Virginia, ²Cardiovascular Research Center,University of Virginia, ³Beirne B. Carter Center for Immunology Research,University of Virginia

Journal (Immunology and Infection)

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Hamad H. Alanazi^1,2, Li She^1,3, Xiao-Dong Li¹

¹Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine,University of Texas Health San Antonio, ²Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,Jouf University, ³Department of Otolaryngology-Head and Neck Surgery,Xiangya Hospital, Central South University

Journal (Immunology and Infection)

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

Dominic Thomas¹, Ting Yu Wu¹, Merylin Cottagiri¹, Maeva Nyandjo¹, Dolores B. Njoku^1,2

¹Department of Anesthesiology and Critical Care Medicine,Johns Hopkins University, ²Department of Pediatrics and Pathology,Johns Hopkins University

Journal (Immunology and Infection)

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

Xue D. Manz¹, Hugo J. Albers^2,3, Petr Symersky⁴, Jurjan Aman¹, Andries D. van der Meer³, Harm Jan Bogaard¹, Robert Szulcek^1,5,6

¹Department of Pulmonary Diseases, Amsterdam UMC,VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), ²BIOS Lab-on-a-Chip group,University of Twente, ³Applied Stem Cell Technologies Group,University of Twente, ⁴Department of Cardio-thoracic Surgery, Amsterdam UMC,VU University Medical Center, ⁵Institute of Physiology,Charité-Universitätsmedizin, ⁶German Heart Center

Journal (Immunology and Infection)

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Sameera Vipat*¹, Greg Brennan*¹, Chorong Park¹, Sherry L. Haller², Stefan Rothenburg¹

¹Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, ²Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Journal (Immunology and Infection)

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Annalise Petriello¹, Jacob A. Becerra², Jason Lay¹, Reem Husaini², Sarah L. Windler¹, Omar Duramad¹, Scot D. Liu¹

¹Department of Research and Development,iQ Biosciences, ²Department of Cellular Products,iQ Biosciences

Journal (Immunology and Infection)

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO⁺ and Light Sheet Fluorescence Microscopy

Laurent Jacob*¹, Jose Brito*^1,2, Jean-Leon Thomas^1,3

¹Université Pierre et Marie Curie Paris, Sorbonne Université,Institut du Cerveau et de la Moelle Epinière, ²Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro, ³Department of Neurology,Yale University School of Medicine

Journal (Immunology and Infection)

Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Kristen E. Funk¹, Sarah K. Lotz¹

¹Department of Biological Sciences,University of North Carolina at Charlotte

Journal (Immunology and Infection)

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Krista D. DiSano¹, Michael R. Linzey^1,2, Nora C. Welsh^1,2, Joshua S. Meier¹, Andrew R. Pachner¹, Francesca Gilli¹

¹Department of Neurology, Geisel School of Medicine,Dartmouth-Hitchcock Medical Center, ²Program in Experimental and Molecular Medicine,Dartmouth College

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