Encyclopedia Of Experiments

Danio rerio (zebrafish)

This collection features research methods using the model organism Danio rerio in its embryo, larva, and adult stages to explore physiological and behavioral questions and create disease models for screening various chemicals.

Encyclopedia Of Experiments

Danio rerio (zebrafish)

This collection features research methods using the model organism Danio rerio in its embryo, larva, and adult stages to explore physiological and behavioral questions and create disease models for screening various chemicals.

Sections

Embryo

Larva

Adult

Embryo

Larva

Adult

Embryo

Embryo-Based Chemical Toxicity Screen

Assessing Effects on Developing Zebrafish Embryos

Mating and Egg Staging

A Method to Generate Embryos and Sort Them by Developmental Stage

Light Sheet Microscopy Sample Preparation

Mounting Live Zebrafish Embryos for Long-Term Imaging

Layered Agar Mounting

Preparing Live Zebrafish Embryos for Long-Term Imaging with an Inverted Microscope

Agar Mounting

A Basic Method of Mounting Live Zebrafish Embryos for Long-Term Imaging

Two-Photon Laser Axotomy

A Method to Injure Axons in Zebrafish Embryos and Observe Axonal Recovery

Whole-Cell Patch Clamp Electrophysiology

A Method to Study Electrical Properties of Neurons

Embryo Microinjection

A Technique to Deliver a Compound into the Zebrafish Yolk

Larva

Photomotor Response Assay

A Method to Measure the Behavioral Response of Larval Zebrafish to a Sudden Change in Lighting Condition

Prey Capture Assay

A Method to Study the Prey Capture Behavior of Zebrafish Larva

Escape Response Assay

A Method to Study the Response of Zebrafish Larva to Touch Stimuli

Zebrafish Avoidance and Thigmotaxis Assay

A High-Throughput Method to Examine Larval Behavior in Response to Aversive Visual Stimulus

Larval Locomotion Assay

High-Throughput Method to Study Neurobehavioral Toxicity of a Compound in Zebrafish Larvae

Targeted Liver Ablation

Inducing Hepatocyte Death with Metronidazole in Transgenic Zebrafish Larvae

Zebrafish Optogenetics

Activating Genetically Modified Somatosensory Neuron to Study Larval Behavioral Responses

Eye Lens Dissection

A Technique to Observe Zebrafish Lens Morphology

Zebrafish Patient-Derived Xenograft (zPDX)

A Cancer Model

Adult

Zebrafish Electrocardiography (ECG)

A Minimally Invasive Assay to Evaluate Cardiac Function

Zebrafish Rheotaxis Assay

A Method for Measuring Orientation Behavior in Response to Different Water Flow Rates

Light-Dark Preference Test

A Method to Study Anxiety-Related Behavior in Zebrafish

Optokinetic Response Assay

A Method to Measure Visual Acuity in Zebrafish

Aggression Assay

A Method to Study Aggressive Behavior of Zebrafish in Response to a Mirror

Novel Tank Diving Assay

A Method to Study Innate Stress Responses in Zebrafish

Morpholino Injection

A Method to Knockdown Target Gene Expression to Study Function in Zebrafish

DNA Extraction from Tail Clip

A Method Used in Zebrafish Genotyping

Adult Zebrafish Electrophysiology

A Method to Measure the Extracellular Electrical Activity of Neurons In Vivo

In Vivo Electroporation

A Site-Specific Method to Transfect Zebrafish

Intramuscular (IM) Injection

Compound Delivery into the Dorsal Muscle of an Adult Zebrafish

Intraperitoneal Injection

A Method of Solution Delivery into the Abdominal Cavity of an Adult Zebrafish

Intrathoracic Injection

Compound Delivery to the Adult Zebrafish Heart

Adult Zebrafish Intestine Dissection

A Technique to Analyze Compound Absorption

Heart Dissection

A Method to Observe Zebrafish Cardiac Development

Zebrafish Brain Dissection

A Technique of Fish Neurobiology

Zebrafish Cardiomyopathy Model

Drug-Induced Cardiotoxicity

Streptozocin Treatment

A Zebrafish Model of Type 1 Diabetes Mellitus