High-Resolution Imaging Technique for Detecting Ca2+ Microdomains in T Cells
1The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology,University Medical Centre Hamburg-Eppendorf, 2Department of Applied Medical Informatics,University Medical Centre Hamburg-Eppendorf, 3Department of Computational Neuroscience,University Medical Centre Hamburg-Eppendorf, 4Department of Immunology,University Medical Centre Hamburg-Eppendorf
Summary
Here, we provide a comprehensive protocol to resolve initial local Ca2+ signals, known as Ca2+ microdomains, in primary murine and human T cells using fluorescence microscopy. This protocol serves as a valuable resource for researchers examining Ca2+ signaling pathways within immune cells and to further unravel their function.
