Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living <em>Drosophila</em> Embryos <em>via</em> Fluorescent Antibody Injection

Qihong Zheng; Xiaoxiang Xiang; Yan Yan; Huapeng  H. Yu

doi:10.3791/66080

Journal

Biologie du développement

Enhancing Protein Visualization with Antibody-Mediated Live Imaging

January 19, 2024

Author Spotlight: Unveiling the Dynamics of Mechanical and Biochemical Signals in Animal Morphogenesis Research

01:35 min

•

475 Views

Fluorescent Labeling of Antibodies for Imaging Low Abundance Proteins in Drosophila

00:49 min

•

61 Views

Preparation and Alignment of Drosophila Embryos for Injecting Fluorescently Labeled Antibodies

02:42 min

•

27 Views

Injecting Fluorescently Labeled Antibodies in Live Drosophila Embryos and Imaging of Low Abundance Proteins

02:21 min

•

42 Views

Qihong Zheng*¹, Xiaoxiang Xiang*¹, Yan Yan^2,3, Huapeng H. Yu¹

¹School of Life Sciences,SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science,Hong Kong University of Science and Technology

Summary

This protocol describes the customized antibody-based fluorescence labeling and injection into early Drosophila embryos to enable live imaging of low-abundance proteins or post-translational modifications that are challenging to detect using traditional GFP/mCherry-tag approaches.