Isolation of Targeted Hypothalamic Neurons for Studies of Hormonal, Metabolic, and Electrical Regulation

Kathera-Ibarra-Forzisi Elena; Christian Roser; Anika Gaur; Federico Sesti

doi:10.3791/65674

Journal

Neurosciences

Hormonal, Metabolic, and Electrical Regulation of Isolated Hypothalamic Neurons

August 04, 2023

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Kathera-Ibarra-Forzisi Elena¹, Christian Roser¹, Anika Gaur¹, Federico Sesti¹

¹Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School,Rutgers University

Summary

Here we present a protocol to grow specific hypothalamic cell subtypes in culture. The cells can be selected based on opportune/unique membrane markers and used in many applications, including immunofluorescence, electrophysiological, and biochemical assays.