Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration

Jacob C. Parson; Nicole K. Biltz; Gretchen A. Meyer

doi:10.3791/65461

Quantification of Skeletal Muscle Fatty Infiltration via Decellularization

June 09, 2023

Author Spotlight: Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration

01:52 min

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Decellularization of Skeletal Muscle

01:43 min

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Intramuscular Adipose Tissue (IMAT) Visualization with Oil Red O Staining

03:25 min

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Estimation of Total Lipid Volume from Decellularized Muscles by Lipid Extraction

01:05 min

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Quantification of Intramuscular Adipose Tissue (IMAT) Lipid Droplet Metrics Using Confocal Imaging

03:22 min

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Jacob C. Parson, Nicole K. Biltz, Gretchen A. Meyer²

¹Program in Physical Therapy,Washington University in St. Louis, ²Departments of Neurology, Orthopaedic Surgery and Biomedical Engineering,Washington University in St. Louis

Summary

The present study describes decellularization-based methodologies for visualizing and quantifying intramuscular adipose tissue (IMAT) deposition through intact muscle volume, as well as quantifying metrics of individual adipocytes that comprise IMAT.