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FRET Analysis of IDR Structural Sensitivity to Study Hyperosmotic Stress in Yeast
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FRETを用いた生細胞の高浸透圧ストレスに応答した内在性変性領域の構造感受性の推定
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JoVE Revista Bioquímica
Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET
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FRETを用いた生細胞の高浸透圧ストレスに応答した内在性変性領域の構造感受性の推定

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01:49 min

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January 12, 2024

DOI:

10.3791/66275-v

DOI:
10.3791/66275-v

•

01:49 min
January 12, 2024

•

8 Views
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1Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

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Intrinsically Disordered RegionsStructural SensitivityHyperosmotic StressFRETConformational EnsembleBiophysical TechniquesCell BiologySaccharomyces CerevisiaeOsmolyteFluorescence Resonance Energy Transfer

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Enriquez-Toledo, C., Ponce-Diego, C. A., Cuevas-Velazquez, C. L. Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET. J. Vis. Exp. (203), e66275, doi:10.3791/66275 (2024).

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