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Análisis fenotípico semiautomatizado de cultivos celulares esferoides funcionales en 3D
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
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Análisis fenotípico semiautomatizado de cultivos celulares esferoides funcionales en 3D

734 Views

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06:48 min

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August 18, 2023

DOI:

10.3791/65086-v

DOI:
10.3791/65086-v

•

06:48 min
August 18, 2023

•

6 Views
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1Department of Biochemistry,Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology,University of Southern Denmark, 3CelVivo ApS

Summary

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Presentamos un protocolo para el cultivo de esferoides de alta reproducibilidad y su caracterización fenotípica mediante captura de imágenes y proteómica.

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3D Cell CultureSpheroidHepG2/C3ATHLE-3Ultra-low Attachment PlateBioreactorProteomic AnalysisLC-MSPhenotypic Analysis

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Cite this Article

Stransky, S., Young, D., Mikkelsen, K., Thulesen, A. P., Frandsen, H. S., Sidoli, S. Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures. J. Vis. Exp. (198), e65086, doi:10.3791/65086 (2023).

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