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Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
 

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Article DOI: 10.3791/65081-v • 07:47 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1
1Department of Molecular Genetics and Cell Biology, The University of Chicago

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This paper describes a method to create wounds in the epithelium of a live Clytia hemisphaerica medusa and image wound healing at a high resolution in vivo. Additionally, a technique to introduce dyes and drugs to perturb signaling processes in the epithelial cells and extracellular matrix during wound healing is presented.

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Epithelial Wound Healing In Vivo Cnidarian Model Organism Clytia Hemisphaerica Visualization Live Animals Complex Environment Reagents Extracellular Matrix Cell Cytoplasm Cell Spreading Cell Migration Mechanisms Of Wound Healing Elizabeth Lee Emily Watto Modified Transfer Pipette Medusa Depression Slide Artificial Seawater (ASW) Micro Wounds Cover Slip Mesoglea
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