Journal
/
Genética
/
A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration
JoVE Revista
Genética
Author Produced
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

DOI:
10.3791/64927-v

•

09:24 min

•

March 10, 2023

•

, , , , ,
1Department of Medical Genetics,University of Wisconsin-Madison, 2McPherson Eye Research Institute,University of Wisconsin-Madison, 3Department of Ophthalmology,Duke University, 4Department of Cell Biology,Duke University

Capítulos

  • 00:04Introduction
  • 01:04Evaluation of Mouse Retinal Pigmented Epithelium (RPE) by Light Microscopy
  • 05:29Evaluation of Mouse RPE Through Confocal Microscopy
  • 06:32Results: Investigating RPE Pathologies in Mice
  • 08:35Conclusion

Summary

Traducción Automática

Traducción Automática

Mouse models can be useful tools for investigating the biology of the retinal pigmented epithelium (RPE). It has been established that mice can develop an array of RPE pathologies. Here, we describe a phenotyping protocol to elucidate and quantify RPE pathologies in mice using light, transmission electron, and confocal microscopy.

Tags

Retinal Pigmented EpitheliumAge-related Macular DegenerationMouse ModelLight Transmission Electron MicroscopyConfocal MicroscopyCardiac PerfusionEye Nucleation

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Simultaneous Video-EEG-ECG Monitoring to Identify Neurocardiac Dysfunction in Mouse Models of Epilepsy

Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Read Article