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使用类器官共培养物对食管成纤维细胞进行功能表征和可视化
JoVE Revista
Biología
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JoVE Revista Biología
Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures
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使用类器官共培养物对食管成纤维细胞进行功能表征和可视化

2,079 Views

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11:58 min

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January 06, 2023

DOI:

10.3791/64905-v

DOI:
10.3791/64905-v

•

11:58 min
January 06, 2023

•

11 Views
, ,
1Department of Cell and Molecular Biology,Karolinska Institutet

Summary

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类器官-成纤维细胞共培养为研究 体内 干细胞生态位提供了一个模型。这里描述了食管类器官-成纤维细胞共培养的方案。此外，全卡口成像用于可视化成纤维细胞-类器官相互作用。

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Eenjes, E., Grommisch, D., Genander, M. Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures. J. Vis. Exp. (191), e64905, doi:10.3791/64905 (2023).

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