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Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures
JoVE 杂志
生物学
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JoVE 杂志 生物学
Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

2,142 Views

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11:58 min

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January 06, 2023

DOI:

10.3791/64905-v

DOI:
10.3791/64905-v

•

11:58 min
January 06, 2023

•

11 Views
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1Department of Cell and Molecular Biology,Karolinska Institutet

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Organoid-fibroblast co-cultures provide a model to study the in vivo stem cell niche. Here, a protocol for esophageal organoid-fibroblast co-cultures is described. Additionally, whole mount imaging is used to visualize the fibroblast-organoid interaction.

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Eenjes, E., Grommisch, D., Genander, M. Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures. J. Vis. Exp. (191), e64905, doi:10.3791/64905 (2023).

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