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Infeksjon av primære nasale epitelceller dyrket ved et luft-væske-grensesnitt for å karakterisere humane koronavirus-vert-interaksjoner
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1Department of Microbiology,University of Pennsylvania, 2Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine,University of Pennsylvania, 3Otorhinolaryngology-Head and Neck Surgery,University of Pennsylvania, 4Corporal Michael J. Crescenz VA Medical Center
Capítulos
- 00:04Introduction
- 00:57Infection of Nasal Air‐Liquid Interface (ALI) Cultures
- 02:53Collection and Quantification of the Shed Virus in Apical Surface Liquid (ASL)
- 03:49Transepithelial Electrical Resistance (TEER) Measurement
- 05:25Measuring Cytotoxicity by Lactate Dehydrogenase (LDH) Assay
- 06:18Results: Characterizing Viral Replication, Host Cell Tropism, and Virus‐Induced Cytotoxicity in Nasal ALI Cultures
- 08:11Conclusion
Neseepitelet er det primære barrierestedet som alle respiratoriske patogener møter. Her skisserer vi metoder for å bruke primære nasale epitelceller dyrket som luft-væske-grensesnitt (ALI) kulturer for å karakterisere humane koronavirus-vert-interaksjoner i et fysiologisk relevant system.
