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Multi-foton laserablation av cytoplasmatiska mikrotubuliorganiseringscentra i musoocyter
JoVE Revista
Biología del desarrollo
This content is Free Access.
JoVE Revista Biología del desarrollo
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
DOI:

08:24 min

November 11, 2022

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Capítulos

  • 00:05Introduction
  • 01:12Mouse Oocyte Collection
  • 02:53Oocyte Microinjection
  • 04:16Oocyte Maturation
  • 05:02Microscope Preparation for Ablation
  • 06:05Ablation of Metaphase Cytoplasmic Microtubule Organizing Centers (mcMTOCs)
  • 06:59Results: Depletion of Metaphase Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
  • 07:35Conclusion

Summary

Traducción Automática

Ett optimerat protokoll presenteras som möjliggör utarmning av cytoplasmatiska mikrotubuliorganiserande centra i musoocyter under metafas I med hjälp av en nära infraröd femtosekundlaser.

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