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A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice

A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice

2,021 Views

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07:17 min

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August 23, 2022

DOI:

10.3791/64309-v

DOI:
10.3791/64309-v

•

07:17 min
August 23, 2022

•

11 Views
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1Department of Radiology,Hackensack University Medical Center, 2Center for Discovery and Innovation,Hackensack Meridian Health, 3Department of Pediatrics,Hackensack University Medical Center, 4Department of Oncology,Georgetown University

Summary

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The present protocol describes an interventional radiology procedure established for intrathymic injection in mice to avoid the risk of open surgery and improve the accuracy of blind percutaneous injections.

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Minimally InvasiveAccurateEfficientIntrathymic InjectionMiceNon-surgicalUltrasound-guidedThymusAtrophyImmunosuppressionAnesthesiaDisinfectionUltrasound SettingsSterile Technique

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Cite this Article

McGuire, M. T., Tuckett, A. Z., Myint, F., Zakrzewski, J. L. A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice. J. Vis. Exp. (186), e64309, doi:10.3791/64309 (2022).

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