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Combinando Microscopia de Limpeza e Fluorescência para Visualização de Mudanças na Expressão Gênica e Respostas Fisiológicas a Plasmodiophora brassicae
JoVE Revista
Biología
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JoVE Revista Biología
Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Combinando Microscopia de Limpeza e Fluorescência para Visualização de Mudanças na Expressão Gênica e Respostas Fisiológicas a Plasmodiophora brassicae

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06:58 min

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August 05, 2022

DOI:

10.3791/64297-v

DOI:
10.3791/64297-v

•

06:58 min
August 05, 2022

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1 Views
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1Integrative Plant Biology Team,Institute of Plant Genetics Polish Academy of Sciences, 2ZMBP-Center for Plant Molecular Biology,University of Tübingen, 3Department of General Botany, Institute of Experimental Biology, Faculty of Biology,Adam Mickiewicz University

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Plasmodiophora BrassicaeClubrootGene ExpressionFluorescence MicroscopyClearingGallsPhytohormoneLignificationPlant-pathogen InteractionAgarose EmbeddingVibratome Sectioning

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Singh, D., Blicharz, S., Stefanowicz, K., Ragni, L., Michalak, K., Bagniewska-Zadworna, A., Malinowski, R. Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae. J. Vis. Exp. (186), e64297, doi:10.3791/64297 (2022).

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