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Organotypic Retinal Explant Cultures from Macaque Monkey
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JoVE Revista Neurociencias
Organotypic Retinal Explant Cultures from Macaque Monkey

Organotypic Retinal Explant Cultures from Macaque Monkey

DOI:
10.3791/64178-v

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10:32 min

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August 24, 2022

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, , , , ,
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University,Yunnan University, 2Institute for Ophthalmic Research,Eberhard-Karls-Universität Tübingen

Capítulos

  • 00:04Introduction
  • 01:05Preparation of Retinal Explants
  • 03:08Primate Retinal Explant Culture
  • 03:36Fixing and Cryo‐Sectioning
  • 05:01Cyclic Guanosine Monophosphate (cGMP) Staining
  • 06:13TUNEL Staining
  • 07:58cGMP Staining Combined with TUNEL Staining
  • 08:40Results: Macaque Monkey Retinal Explant Culture
  • 09:20Conclusion

Summary

Traducción Automática

Traducción Automática

Retinal explants obtained from wild-type macaques were cultured in vitro. Retinal degeneration and the cGMP-PKG signaling pathway was induced using the PDE6 inhibitor zaprinast. cGMP accumulation in the explants at different zaprinast concentrations was verified using immunofluorescence.

Tags

Organotypic Retinal ExplantMacaque MonkeyRetinal DegenerationIn Vivo ConditionsAnimal Use ReductionRetinal DevelopmentRetinal Explant PreparationBacterial ContaminationRetinal Explant CultureDrug TreatmentCryo-sectioningParaformaldehyde Fixation

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