Journal
/
Investigación sobre el cáncer
/
Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
JoVE Revista
Investigación sobre el cáncer
This content is Free Access.
JoVE Revista Investigación sobre el cáncer
Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

3,080 Views

•

13:01 min

•

June 03, 2022

DOI:

10.3791/64171-v

DOI:
10.3791/64171-v

•

13:01 min
June 03, 2022

•

10 Views
, , , , , , , ,
1Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology,Uniformed Services University and Walter Reed National Military Medical Center, 2The Henry M. Jackson Foundation for the Advancement of Military Medicine, 3The John P. Murtha Cancer Center Research Program, Department of Surgery,Uniformed Services University, 4Women’s Health Integrated Research Center, Inova Women’s Service Line,Inova Health System

Summary

Automatically generated

This protocol describes a high-throughput workflow for artificial intelligence-driven segmentation of pathology-confirmed regions of interest from stained, thin tissue section images for enrichment of histology-resolved cell populations using laser microdissection. This strategy includes a novel algorithm enabling the transfer of demarcations denoting cell populations of interest directly to laser microscopes.

Explore More Videos

AI-driven Image AnalysisLaser MicrodissectionProteomic AnalysisTumor MicroenvironmentPathomicsCalibration FiducialsPEN MembraneImage Analysis SoftwareAnnotationsPartitioningTiled AnnotationsPython Dapa Algorithm

Read Article

Cite this Article

Mitchell, D., Hunt, A. L., Conrads, K. A., Hood, B. L., Makohon-Moore, S. C., Rojas, C., Maxwell, G. L., Bateman, N. W., Conrads, T. P. Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment. J. Vis. Exp. (184), e64171, doi:10.3791/64171 (2022).

Download .ris file

Copy

Share Video

.

Copy