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JoVE Journal Genetics
Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing
 

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.

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Knock-Out Muscle Cell Lines Lentivirus-Mediated CRISPR/Cas9 Gene Editing Muscle Cell Knock-out Gene Function Cheap And Faster Knock-out Animals Myopathy IPS Cells CRISPR-mediated Gene Deletion Genome Browser Tools Target Gene DNA Sequences Gene Exons Guide RNA Nucleotide Sequences
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