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检测胎儿组织来源肠的上皮通透性

JoVE Revista
Medicina

This content is Free Access.

JoVE Revista Medicina

Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids

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检测胎儿组织来源肠的上皮通透性

1,779 Views

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07:51 min

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June 16, 2022

DOI:

10.3791/64108-v

DOI:

10.3791/64108-v

•

07:51 min

June 16, 2022

•

5 Views

Amelia Llerena¹, Shaheda Urmi², Jahanshah Amin³, Byeong Cha³, Thao TB Ho²

¹Morsani College of Medicine,University of South Florida, ²Department of Pediatrics, Morsani College of Medicine,University of South Florida, ³Department of Molecular Pharmacology and Physiology, Morsani College of Medicine,University of South Florida

Summary

该协议详细介绍了从胎儿肠道组织建立肠样，一种三维肠道模型。上皮生物标志物的免疫荧光成像用于模型表征。脂多糖（一种细菌内毒素）的顶端暴露，使用显微注射技术以剂量依赖性方式诱导上皮通透性，通过荧光葡聚糖的泄漏来测量。

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