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Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
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Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
Capítulos
- 00:04Introduction
- 00:34Micromilling
- 03:24Channel Sealing
- 04:38Device Preparation and Microparticle Trap Formation
- 05:32Experimental Mounting and Immunodetection
- 07:42Results: Indirect Immunoassay with Enzyme-Labeled Antibody-Based Detection
- 09:14Conclusion
Microfluidics is a powerful tool for the development of diagnostic tests. However, expensive equipment and materials, as well as laborious fabrication and handling techniques, are often required. Here, we detail the fabrication protocol of an acrylic microfluidic device for magnetic micro- and nanoparticle-based immunoassays in a low-cost and simple-to-use setting.
