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Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections
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JoVE Revista Neurociencias
Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections

Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections

3,030 Views

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10:53 min

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April 20, 2022

DOI:

10.3791/63804-v

DOI:
10.3791/63804-v

•

10:53 min
April 20, 2022

•

34 Views
,
1Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Cell Biology and Physiology,University of North Carolina, Chapel Hill

Summary

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This protocol describes methods for sectioning, staining, and imaging free-floating tissue sections of the mouse brain, followed by a detailed description of the analysis of astrocyte territory volume and astrocyte territory overlap or tiling.

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Cite this Article

Eaker, A. R., Baldwin, K. T. Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections. J. Vis. Exp. (182), e63804, doi:10.3791/63804 (2022).

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