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Neuroscience

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JoVE Journal Neuroscience
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
 

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Article DOI: 10.3791/63714-v • 05:40 min • May 5th, 2022
May 5th, 2022 •
*1, *1, 1
1Australian Institute for Bioengineering and Nanotechnology, The University of Queensland
* These authors contributed equally

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In this study, we provide a detailed technique for a simple yet robust cortical organoid culture system using standard feeder-free hPSC cultures. This is a rapid, efficient, and reproducible protocol for generating organoids that model aspects of brain senescence in vitro.

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Cortical Brain Organoids Neuronal Senescence In Vitro Modeling Robustness Reproducibility HPSCs Neuroectodermal Colonies Neuroepithelial Spheroids Tissue Architecture Senescence Markers Aging-related Neuronal Processes N2 Medium Dual SMAD Inhibitors 2D To 3D Conversion Dispase Colony Detachment
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