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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
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Bioquímica
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JoVE Revista Bioquímica
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

DOI:
10.3791/63668-v

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11:38 min

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April 05, 2022

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, , ,
1Department of Chemistry,University of San Francisco, 2Department of Chemistry,University of California, Davis

Capítulos

  • 00:04Introduction
  • 00:59Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
  • 02:44Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
  • 03:56Isothermal Titration Calorimetry
  • 09:00Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
  • 10:48Conclusion

Summary

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Traducción Automática

This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

Tags

Cu(II)PeptideMetal-peptide InteractionsElectronic Absorption SpectroscopyBufferCuvetteAbsorption SpectrumTitration

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