Journal
/
Bioquímica
/
Un test di pull-down a singola molecola ottimizzato per la quantificazione della fosforilazione delle proteine
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Please note that all translations are automatically generated. Click here for the English version.

Un test di pull-down a singola molecola ottimizzato per la quantificazione della fosforilazione delle proteine

2,553 Views

•

07:45 min

•

June 06, 2022

DOI:

10.3791/63665-v

DOI:
10.3791/63665-v

•

07:45 min
June 06, 2022

•

7 Views
, , , , , , ,
1Department of Pathology,University of New Mexico School of Medicine, 2Department of Physics and Astronomy,University of New Mexico, 3Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Tags

AI-powered Protein Phosphorylation QuantificationSingle-molecule Pull-down AssaySiMPull ProtocolAntibody LabelingAutofluorescence ReductionSingle-molecule AnalysisPhosphorylation Status Of Individual ProteinsAlternative To Western Blotting And Mass Spectrometry

Read Article

Cite this Article

Bailey, E. M., Salazar-Cavazos, E., Grattan, R. M., Wester, M. J., Schodt, D. J., Rojo, J. A., Lidke, K. A., Lidke, D. S. An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation. J. Vis. Exp. (184), e63665, doi:10.3791/63665 (2022).

Download .ris file

Copy

Share Video

.

Copy