Müller glia primary cultures obtained from mouse retinas represent a very robust and reliable tool to study the glial conversion into retinal progenitor cells after microRNA treatment. Single molecules or combinations can be tested before their subsequent application of in vivo approaches.
Kang, S., Wohl, S. Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment. J. Vis. Exp. (181), e63651, doi:10.3791/63651 (2022).