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Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
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Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
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08:07 min
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March 24, 2023
DOI:
1Centro de Investigaciones en Óptica A.C. (CIO), 2Division of Sciences and Engineering, Department of Chemical, Electronic, and Biomedical Engineering,Universidad de Guanajuato, 3Institute of Physiology,Benemérita Universidad Autónoma de Puebla, 4Dirección General de Desarrollo Internacional,Benemérita Universidad Autónoma de Puebla
Summary
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In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.
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Piazza, V., Alata, M., Hernandez, V. H., Eguibar, J. R., Cortes, C. Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin. J. Vis. Exp. (193), e63449, doi:10.3791/63449 (2023).
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