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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
JoVE Revista
Bioingeniería
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JoVE Revista Bioingeniería
Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

DOI:
10.3791/63403-v

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13:21 min

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April 06, 2022

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, , , ,
1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences,Ghent University, 2Health and Happiness (H&H) Group,National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry,Graz University of Technology

Capítulos

  • 00:04Introduction
  • 00:48Preparing Micro-Patterned Agarose Coated Tissue Culture Plate
  • 02:27Preparing Oxygen Probe-Loaded Spheroids
  • 03:50Spheroids Bioprinting Procedure
  • 05:48Preparation of Spheroids for Live Imaging Analysis
  • 07:10Image Acquisition and Presentation
  • 10:22Results: Kinetic Study of Oxygen Probe and Comparative Analysis of Spheroids
  • 12:18Conclusion

Summary

Traducción Automática

Traducción Automática

The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.

Tags

Oxygen MicroscopyBiofabricationMulticellular Spheroids3D PrintingNear-infrared Oxygen ProbePDMS StampsAgarose Micro-patterningCell CultureSpheroid Formation

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