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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
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JoVE Revista Medicina
Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

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08:48 min

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February 16, 2022

DOI:

10.3791/63352-v

DOI:
10.3791/63352-v

•

08:48 min
February 16, 2022

•

4 Views
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1Department of Anatomy & Cell Biology,Rush University Medical Center, 2Department of Microbial Pathogens and Immunity,Rush University Medical Center, 3Department of Pathology,Rush University Medical Center

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This article describes a convenient method for monitoring dynamic changes in antibody titers for two immunoglobulin isotypes concurrently (either IgA, IgM, or IgG) resulting from an immune response to SARS-CoV-2 infection or vaccination. This 'Multianalyte Covid-19 Immune Response Panel' employs three indirect immunoassays built on coded microspheres that are read using a flow-based multiplex reader with 'dual-channel' capability.

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SeroconversionMultianalyte Immunobead AssayCOVID-19Antibody DetectionHigh-throughputEpitope EvaluationCell SignalingImmune Response MonitoringMagnetic MicrospheresBead ActivationProtein CouplingMES BufferEDCNHS

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Fhied, C. L., Tarhoni, I., Gerard, D., Lewin, G. M., Moudgalya, H., Schneider, J. R., Borgia, J. A. Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19. J. Vis. Exp. (180), e63352, doi:10.3791/63352 (2022).

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