The present work describes the steps for producing ready-to-use qPCR for T. cruzi DNA detection that can be pre-loaded on the reaction vessel and stored in the refrigerator for several months.
Costa, A. D. T., Amadei, S. S., Bertão-Santos, A., Rodrigues, T. Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms. J. Vis. Exp. (179), e63316, doi:10.3791/63316 (2022).