Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
使用共聚焦显微镜以高时间分辨率记录小鼠神经肌肉接头中的钙瞬变
1,753 Views
•
11:12 min
•
December 01, 2021
DOI:
1Laboratory of Biophysics of Synaptic Processes,Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2Department of Radiophotonics and Microwave Technologies,Kazan National Research Technical University named after A.N. Tupolev, 3Department of Normal Physiology,Kazan State Medical University
Summary
Automatically generated
该方案描述了通过切割神经将荧光钙染料加载到小鼠运动神经末梢的方法。此外，还提出了一种使用共聚焦显微镜记录周围神经末梢快速钙瞬变的独特方法。
Read Article
Cite this Article
Zhilyakov, N. V., Arkhipov, A. Y., Khaziev, E. F., Mukhamedyarov, M. A., Samigullin, D. V. Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy. J. Vis. Exp. (178), e63308, doi:10.3791/63308 (2021).
Copy