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Isolamento de Células Endoteliais Primárias de Pulmão de Camundongo

JoVE Revista
Inmunología y contagio

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JoVE Revista Inmunología y contagio

Isolation of Primary Mouse Lung Endothelial Cells

Isolamento de Células Endoteliais Primárias de Pulmão de Camundongo

3,705 Views

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06:41 min

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November 10, 2021

DOI:

10.3791/63253-v

DOI:

10.3791/63253-v

•

06:41 min

November 10, 2021

•

15 Views

Erika Wong², Nina Nguyen, Judith Hellman

¹Department of Pediatrics, Division of Critical Care,UCSF Benioff Children’s Hospital, ²Department of Anesthesia and Perioperative Care,University of California, San Francisco

Tags

Primary Mouse Lung Endothelial Cells Isolation Cell Culture CD31 CD102 Immunobeads Collagenase HBSS DMEM Cell Strainer Trituration

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