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Developmental Biology

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JoVE Journal Developmental Biology
Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae
 

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Article DOI: 10.3791/63251-v • 05:03 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1, 1, 2,3, 1
1Department of Toxicology, School of Public Health, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Medical College of Soochow University, 2Department of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Control and Prevention, 3Jiangsu Preventive Medicine Association, Nanjing, China
* These authors contributed equally

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Here, we have used alizarin red staining to show that lead acetate exposure causes a bone mass change in zebrafish larvae. This staining method can be adapted to the investigation of bone loss in zebrafish larvae loss induced by other hazardous toxicants.

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Alizarin Red Staining Bone Loss Zebrafish Larvae Chemical-induced Toxicity Skeletal Changes Osteoporosis Bone Mass Fixation Paraformaldehyde 1X PBS Decantation Tris HCL Magnesium Chloride De-staining Bleaching Hydrogen Peroxide Potassium Hydroxide Glycerin Alizarin Staining
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