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Generazione di organoidi cerebrali da cellule staminali pluripotenti indotte in mini bioreattori fatti in casa
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Generazione di organoidi cerebrali da cellule staminali pluripotenti indotte in mini bioreattori fatti in casa
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10:16 min
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December 11, 2021
DOI:
1Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics,Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 4Department of Pathological Anatomy,North-Western State Medical University, 5Department of Biology,Lomonosov State University
Summary
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Qui descriviamo un protocollo per la generazione di organoidi cerebrali da cellule staminali pluripotenti indotte umane (iPSC). Per ottenere organoidi cerebrali in grandi quantità e di alta qualità, utilizziamo mini bioreattori fatti in casa.
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Cite this Article
Eremeev, A., Belikova, L., Ruchko, E., Volovikov, E., Zubkova, O., Emelin, A., Deev, R., Lebedeva, O., Bogomazova, A., Lagarkova, M. Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors. J. Vis. Exp. (178), e62987, doi:10.3791/62987 (2021).
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