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An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila
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An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

1,956 Views

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07:06 min

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October 13, 2021

DOI:

10.3791/62983-v

DOI:
10.3791/62983-v

•

07:06 min
October 13, 2021

•

4 Views
,
1Department of Biology, Howard Hughes Medical Institute, Department of Biology,Stanford University

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This protocol describes the dissection procedure, culture condition, and live imaging of an antennae-brain explant system for the study of the olfactory circuit assembly.

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Olfactory CircuitTime-lapse ImagingDrosophilaExplant SystemBrain DissectionMicrodissectionPupaeCulture MediumSylgard PlateMicro PinsHeat ShockSchneider’s Drosophila MediumFetal Bovine SerumHuman Insulin20-hydroxyecdysone

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Li, T., Luo, L. An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila. J. Vis. Exp. (176), e62983, doi:10.3791/62983 (2021).

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