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Dual-Color Fluorescence Cross-Correlation Spectroscopy om eiwit-eiwitinteractie en eiwitdynamica in levende cellen te bestuderen
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Capítulos
- 00:05Introduction
- 01:09Sample Preparation
- 04:34Calibration Measurements
- 05:58Live Cell Measurements
- 06:52Data Export
- 07:23Calibration Measurements
- 09:20Live Cell Experiments
- 11:20Resultados
- 13:17Conclusion
We presenteren een experimenteel protocol en data-analyseworkflow om live cell dual-color fluorescentie cross correlation spectroscopie (FCCS) uit te voeren in combinatie met Förster Resonance Energy transfer (FRET) om membraanreceptordynamica in levende cellen te bestuderen met behulp van moderne fluorescentielabeltechnieken.
Tags
Keywords:
Fluorescence Correlation SpectroscopyCross-correlation SpectroscopyFRETG Protein-coupled ReceptorsBeta-2 Adrenergic ReceptorsSphingolipid ReceptorsProtein-protein InteractionProtein DynamicsLive CellsCell CultureTransfectionSNAP-tagFluorescence AnisotropyTranslational DiffusionRotational Diffusion
