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基于荧光的连续核酸内切酶偶联DNA甲基化测定，用于筛选DNA甲基转移酶抑制剂
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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
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基于荧光的连续核酸内切酶偶联DNA甲基化测定，用于筛选DNA甲基转移酶抑制剂

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06:07 min

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August 05, 2022

DOI:

10.3791/62949-v

DOI:
10.3791/62949-v

•

06:07 min
August 05, 2022

•

5 Views
, ,
1Department of Chemistry,Bucknell University, 2Program in Cell Biology/Biochemistry,Bucknell University

Summary

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DNA甲基转移酶是潜在的癌症药物靶标。在这里，提出了一个协议来评估DNA甲基转移酶抑制的小分子。该测定利用核酸内切酶将DNA甲基化与荧光生成偶联，并允许实时监测酶活性。

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Switzer, R., Ward, K. A., Medrano, J. Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors. J. Vis. Exp. (186), e62949, doi:10.3791/62949 (2022).

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