Journal
/
Biología del desarrollo
/
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

DOI:
10.3791/62930-v

•

08:16 min

•

August 13, 2021

•

, , ,
1Department of Biology,Indiana University of Pennsylvania

Capítulos

  • 00:04Introduction
  • 00:32Raising Larvae
  • 01:03Days 1-2: Fixing Larvae and Day 3: Measuring Larvae
  • 02:16Days 3 4: Dehydration and Day 5: Rehydration, Proteinase K Digestion, Bleaching and Prehybridization
  • 04:05Day 6: Probe Hybridization and Day 7: Probe Washing and Blocking
  • 05:38Days 8 9: Antibody Incubation and Days 10 11: Antibody Washing
  • 06:05Day 12: Staining and Imaging
  • 07:18Results: In Situ Hybridization for Gene Expression Analysis in Zebrafish Larvae and Juveniles During Mesonephros Development
  • 07:52Conclusion

Summary

Traducción Automática

Traducción Automática

The zebrafish is an important model for understanding kidney development. Here, an in situ hybridization protocol is optimized to detect gene expression in zebrafish larvae and juveniles during mesonephros development.

Tags

In Situ HybridizationZebrafish LarvaeZebrafish JuvenilesMesonephros DevelopmentGene ExpressionProbe PenetrationKidney VisualizationMatingEmbryo CollectionLarva FixationLarva DehydrationLarva RehydrationProteinase K DigestionBleaching

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Induction of Hypoxia in Living Frog and Zebrafish Embryos

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Wholemount In Situ Hybridization for Astyanax Embryos

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Read Article