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Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
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Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
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August 06, 2021
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1Integrated Physiology and Molecular Medicine Laboratory,Pennington Biomedical Research Center, 2Clinical Oncology and Metabolism,Pennington Biomedical Research Center, 3Department of Nutrition,Case Western Reserve University, 4Department of Clinical Nutrition,University of Texas Southwestern Medical Center
Summary
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We developed a practical protocol and analytical approach to evaluate mitochondrial oxidative phosphorylation and electron transfer capacity in fresh tumor homogenates. This protocol can be easily adapted to survey various mitochondrial functions that contribute to cancer initiation, progression, and treatment response.
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Zunica, E. R. M., Axelrod, C. L., Gilmore, L. A., Kirwan, J. P. Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates. J. Vis. Exp. (174), e62875, doi:10.3791/62875 (2021).
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