Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
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August 16, 2021
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Preparation of mitochondria-enriched samples from previously frozen archived solid tissues allowed the investigators to perform both functional and analytical assessments of mitochondria in various experimental modalities. This study demonstrates how to prepare mitochondria-enriched preparations from frozen heart tissue and perform analytical assessments of mitochondria.
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Barrera, D., Upton, S., Rauch, M., Notarianni, T., Eum, K. S., Liberty, M., Sah, S. V., Liu, R., Newcomer, S., May, L. E., Agbas, E., Sage, J., Kosa, E., Agbas, A. Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes. J. Vis. Exp. (174), e62809, doi:10.3791/62809 (2021).
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