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使用同质珠为基础的检测的查佩龙-科查佩龙相互作用研究
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JoVE Revista Bioquímica
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
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使用同质珠为基础的检测的查佩龙-科查佩龙相互作用研究

2,544 Views

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06:51 min

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July 21, 2021

DOI:

10.3791/62762-v

DOI:
10.3791/62762-v

•

06:51 min
July 21, 2021

•

5 Views
, , , ,
1Department of Neuroscience Care and Society, Division of Neurogeriatrics,Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology,Indian Institute of Technology (BHU), 3Theme Inflammation and Aging,Karolinska University Hospital

Summary

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该协议提出了一种利用谷胱甘肽相关的供体珠与 GST 融合的 TPR-motif 共同伴奏和接受珠以及 Hsp90 衍生肽来探索蛋白质- 蛋白质相互作用的技术。我们使用此技术筛选小分子，以破坏 Hsp90-FKBP51 或 Hsp90-FKBP52 相互作用，并识别出有效和选择性的 Hsp90-FKBP51 相互作用抑制剂。

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Cite this Article

Wang, L., Bergkvist, L., Kumar, R., Winblad, B., Pavlov, P. F. Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay. J. Vis. Exp. (173), e62762, doi:10.3791/62762 (2021).

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