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Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira
JoVE Revista
Genética
Author Produced
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JoVE Revista Genética
Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

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14:49 min

August 14, 2021

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14:49 min
August 14, 2021

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Summary

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Here, the application of CRISPR interference (CRISPRi) for specific gene silencing in Leptospira species is described. Leptospira cells are transformed by conjugation with plasmids expressing dCas9 (catalytically "dead" Cas9) and a single-guide RNA (sgRNA), responsible for base pairing to the desired genomic target. Methods to validate gene silencing are presented.

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